Join us   Log in  


Pages: 22-30
Print Article   Download XML  Download PDF

Tea Root Extract (Camellia sinensis) and its tritepenoidsaponin induce apoptosis and cell cycle arrest in HL60 cell lines .

Author: Dr.PapiyaGhosh( neeChatterjee)

Category: Engineering, Science and Mathematics


The presence of triterpenoidsaponins in tea root has been reported.The anticancer activity of this extract on U937 and K562 cell lines had been previously reported by us. However, no studies concerning biological activity of the plant extracts on HL60 cells have been done so far. In the present study, the anti-leukemic activity of the methanol extract of tea root and two of its saponins TS1 and TS2 have been investigated, and efforts have been made to understand the molecular cause of the such positive effect. The effect of the respective compounds on normal blood cells have been also estimated. The anti-leukemic activity of ME(methanol extract), TS1 and TS2, was investigated on leukemic cells using cell viability and MTT assays. The molecular pathways leading to the activity of tea root extract ,TS1 and TS2 were examined by confocal microscopy and flow-cytometric analysis. Tea root extract and its saponins TS1 and TS2 inhibited cell growth and produced significant cytotoxicity in HL60 cells. TRE and its compound produced apoptotic cell death which resulted in the subsequent accumulation of cells in the sub-G0 phase followed by DNA fragmentation.Tea root extract and its triterpenoidsaponins possesses significant anti-leukemic activity on HL60 cells . Since the necrotic body formation is low after treatment and apoptosis was not induced in normal cell, the occurrence of inflammation in in vivo systems could be reduced, which represents a positive indication in view of therapeutic application.

Keywords: promyelocytic leukemia; Tea Root Extract; TriterpenoidSaponins; Apoptosis; Cell Cycle Arrest